@article{6171,
  keywords = {CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cytokines, Cytoplasm, Flow Cytometry, Humans, Interferon-gamma, Interleukin-10, Interleukin-4, leprosy, Mycobacterium leprae, Tuberculin, Tuberculosis, Pulmonary, Tumor Necrosis Factor-alpha},
  author = {Antas P R Z and Sales J S and Pereira K C and Oliveira E B and Cunha K S and Sarno E N and Sampaio E P},
  title = {Patterns of intracellular cytokines in CD4 and CD8 T cells from patients with mycobacterial infections.},
  abstract = {<p>Using a short-term bulk culture protocol designed for an intracellular-staining method based on a flow cytometry approach to the frequencies of cytokine-producing cells from tuberculosis and leprosy patients, we found distinct patterns of T cell subset expression. The method also reveals the profile of peak cytokine production and can provide simultaneous information about the phenotype of cytokine-producing cells, providing a reliable assay for monitoring the immunity of these patients. The immune response of Mycobacterium leprae and purified protein derivative (PPD) in vitro to a panel of mycobacteria-infected patients from an endemic area was assessed in primary mononuclear cell cultures. The kinetics and source of the cytokine pattern were measured at the single-cell level. IFN-gamma-, TNF-alpha-, IL-4- and IL-10-secreting T cells were intracytoplasmic evaluated in an attempt to identify M. leprae- and PPD-specific cells directly from the peripheral blood. The analysis by this approach indicated that TNF-alpha was the first (8 h) to be produced, followed by IFN-gamma (16 h), IL-10 (20 h) and IL-4 (24 h), and double-staining experiments confirmed that CD4+ were a greater source of TNF-alpha than of CD8+ T cells (P < 0.05). Both T cell subsets secreted similar amounts of IFN-gamma. We conclude that the protocol permits rapid evaluation of cytokine production by different T cell populations. The method can also be used to define immune status in non-infected and contact individuals.</p>},
  year = {2004},
  journal = {Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas},
  volume = {37},
  pages = {1119-29},
  month = {2004 Aug},
  issn = {0100-879X},
  doi = {10.1590/s0100-879x2004000800003},
  language = {eng},
}