@article{6119,
  keywords = {Granuloma, Humans, Immunohistochemistry, leprosy, Macrophages, Microscopy, Immunoelectron, Mycobacterium leprae, Nitric Oxide Synthase, Peripheral nerves, S100 Proteins, Skin, Skin Diseases, Bacterial, Tyrosine},
  author = {Schön T and Hernández-Pando R and Baquera-Heredia J and Negesse Y and Becerril-Villanueva L E and Eon-Contreras J C L and Sundqvist T and Britton S},
  title = {Nitrotyrosine localization to dermal nerves in borderline leprosy.},
  abstract = {<p><b>BACKGROUND: </b>Nerve damage is a common and disabling feature of leprosy, with unclear aetiology. It has been reported that the peroxidizing agents of myelin lipids-nitric oxide (NO) and peroxynitrite-are produced in leprosy skin lesions.</p><p><b>OBJECTIVES: </b>To investigate the localization of nitrotyrosine (NT)-a local end-product of peroxynitrite-in leprosy lesions where dermal nerves are affected by a granulomatous reaction.</p><p><b>METHODS: </b>We investigated by immunohistochemistry and immunoelectron microscopy the localization of the inducible NO synthase (iNOS) and NT in biopsies exhibiting dermal nerves from patients with untreated leprosy.</p><p><b>RESULTS: </b>There were abundant NT-positive and iNOS-positive macrophages in the borderline leprosy granulomas infiltrating peripheral nerves identified by light microscopy, S-100 and neurofilament immunostaining. Immunoelectron microscopy showed NT reactivity in neurofilament aggregates and in the cell wall of Mycobacterium leprae.</p><p><b>CONCLUSIONS: </b>Our results suggest that NO and peroxynitrite could be involved in the nerve damage following borderline leprosy.</p>},
  year = {2004},
  journal = {The British journal of dermatology},
  volume = {150},
  pages = {570-4},
  month = {2004 Mar},
  issn = {0007-0963},
  doi = {10.1046/j.1365-2133.2004.05764.x},
  language = {eng},
}