@article{3964,
  keywords = {Amino Acid Sequence, Antibodies, Monoclonal, Antigens, Bacterial, Base Sequence, Cells, Cultured, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Genes, Humans, Immunity, Cellular, In Vitro Techniques, Leprosy, Tuberculoid, Lymphocyte Activation, Molecular Sequence Data, Mycobacterium leprae, Oligodeoxyribonucleotides, Receptors, Antigen, T-Cell, alpha-beta, Sequence Alignment, T-Lymphocytes},
  author = {Schooten W C and Ko J L and Stoep N and Haanen J B and Pickering L and Vries R R and Elsen P},
  title = {T-cell receptor beta-chain gene usage in the T-cell recognition of Mycobacterium leprae antigens in one tuberculoid leprosy patient.},
  abstract = {<p>The beta chain of the T-cell antigen receptor present on 20 T-cell clones isolated from a tuberculoid leprosy patient was studied by gene rearrangement and PCR analysis. These T-cell clones all responded to Mycobacterium leprae-encoded protein antigens, and 8 of them specifically recognized peptides of the mycobacterial 65-kDa heat shock polypeptide (65hsp). All T-cell clones studied were HLA-DR-restricted (DR2 or -3). In the DR3-restricted group, 7 of 10 used a beta-chain variable region V beta 5 gene family member, whereas in the DR2-restricted group, 2 of 10 T-cell clones used a V beta 5 gene segment and 5 used the V beta 18 gene segment. The deduced amino acid sequences of the beta chain from 8 T-cell clones have revealed that 3 of 4 DR3-restricted T-cell clones expressed the V beta 5.1 gene segment whereas the fourth DR3-restricted T-cell clone employed a V beta 5 family member not previously described. The V beta 5.1-positive T-cell clones all recognized the same 65hsp peptide from residues 2 to 12. The N-D-N segment (where D is diversity) of the junctional region of these T-cell clones was very similar, despite different beta-chain joining gene segments. Of the 4 DR2-restricted T-cell clones investigated, 3 used the V beta 18 gene segment and recognized the 65hsp peptide from residues 418 to 427. In conclusion, within this panel of M. leprae-reactive T-cell clones, the DR3-restricted T-cell clones mainly used a V beta 5 gene segment, whereas the DR2-restricted clones employed preferentially the V beta 18 gene segment.</p>},
  year = {1992},
  journal = {Proceedings of the National Academy of Sciences of the United States of America},
  volume = {89},
  pages = {11244-8},
  month = {1992 Dec 01},
  issn = {0027-8424},
  doi = {10.1073/pnas.89.23.11244},
  language = {eng},
}