@article{371,
  keywords = {Adolescent, Adult, Aged, Animals, Child, Child, Preschool, DNA, Protozoan, Ethiopia, Female, Humans, Infant, Isoenzymes, Leishmania, Leishmaniasis, Cutaneous, Male, Middle Aged, polymerase chain reaction, Polymorphism, Restriction Fragment Length, Species Specificity},
  author = {Gadisa E and Genetu A and Kuru T and Jirata D and Dagne K and Aseffa A and Gedamu L},
  title = {Leishmania (Kinetoplastida): species typing with isoenzyme and PCR-RFLP from cutaneous leishmaniasis patients in Ethiopia.},
  abstract = {<p>Cutaneous leishmaniasis (CL) is an increasing public health problem in Ethiopia. There is a concern that it is spreading with increased incidence. In this study, we used isoenzyme electrophoresis and internal transcribed spacer one (ITS1) PCR-RFLP techniques to identify Leishmania species from CL patients in Ethiopia. We obtained isolates from 55 localized cutaneous leishmaniasis (LCL), 3 diffused cutaneous leishmaniasis (DCL) and 36 biopsy samples from 34 LCL and 2 DCL cases from All Africa Leprosy and Tuberculosis Rehabilitation and Training Center (ALERT) and clinically diagnosed CL cases from Ochollo village. Both isoenzyme and ITS1 PCR-RFLP techniques showed that Leishmania aethiopica (L. aethiopica) was the aetiologic agent in all cases. Our study also showed that ITS1 PCR-RFLP could identify Leishmania species from biopsy samples and suggests the method could be used for epidemiological surveillance of leishmaniasis in Ethiopia and for species-specific diagnosis.</p>},
  year = {2007},
  journal = {Experimental parasitology},
  volume = {115},
  pages = {339-43},
  month = {2007 Apr},
  issn = {0014-4894},
  doi = {10.1016/j.exppara.2006.09.014},
  language = {eng},
}