@article{3475,
  keywords = {Antibodies, Bacterial, Antigens, Bacterial, Arthritis, Rheumatoid, Buffers, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, Immunoglobulin G, leprosy, Lyme Disease, Mycobacterium leprae, Sensitivity and Specificity, Tromethamine},
  author = {Sticht-Groh V and Alvarenga A E and Vettom L and Ballestrem W},
  title = {Use of a different buffer system in the phenolic glycolipid-I ELISA.},
  abstract = {<p>By changing the buffer system in the phenolic glycolipid-I (PGL-I) enzyme-linked immunosorbent assay (ELISA) the sensitivity of the test was increased without altering its specificity. Using a Tris-HCl buffer, significant titers of &gt; or = 1:300 were found in 53.1% of the sera in paucibacillary (PB) and 98.0% of the sera in multibacillary (MB) groups of patients. Titer levels were also significantly increased. In the PB group of patients with Tris-HCl, the highest titer detected was 1:1200; in the MB group of patients, 1:76,800. Through this modification of the buffer system a more sensitive test was obtained thereby increasing the detectable level of PGL-I antibodies in both the PB and MB groups of patients.</p>
},
  year = {1992},
  journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  volume = {60},
  pages = {570-4},
  month = {1992 Dec},
  issn = {0148-916X},
  url = {http://ila.ilsl.br/pdfs/v60n4a07.pdf},
  language = {eng},
}