@article{3358, keywords = {Animals, Antigens, Protozoan, Concanavalin A, Electrophoresis, Polyacrylamide Gel, Epitopes, Humans, Leishmania donovani, Leishmaniasis, Visceral, Serologic Tests}, author = {Bhattacharya M and Banerjee N and Ghosh D K}, title = {Leishmania donovani: isolation of a concanavalin-A specific antigen and its evaluation for serodiagnosis of visceral leishmaniasis.}, abstract = {

A glycoconjugate antigen of 27-39 kDa was isolated from a cell-free extract of Leishmania donovani by affinity chromatography using a Concanavalin-A sepharose-4B column and eluted with 0.5 M alpha-methylmannoside. The antigen was recognized specifically by sera from kala-azar (visceral leishmaniasis) patients and did not react with sera from tuberculosis, leprosy or malaria patients. The antigen may therefore be useful in developing a serodiagnostic assay for visceral leishmaniasis.

}, year = {1992}, journal = {Annals of tropical medicine and parasitology}, volume = {86}, pages = {341-5}, month = {1992 Aug}, issn = {0003-4983}, doi = {10.1080/00034983.1992.11812676}, language = {eng}, }