@article{32158,
  author = {Tarique M and Naz H and Kurra S and Saini C and Naqvi RA and Rai R and Suhail M and Khanna N and Rao DN and Sharma A},
  title = {Interleukin-10 Producing Regulatory B Cells Transformed CD4CD25 Into Tregs and Enhanced Regulatory T Cells Function in Human Leprosy.},
  abstract = {<p>Regulatory B cells (Bregs) are known to exhibit their regulatory functions through interleukin-10 (IL-10) cytokine which suppress inflammation. There are only a few studies explaining the phenotype and functioning of these cells in contribution to host immunity in leprosy. Here, we evaluated the role of IL-10 producing Bregs in the pathogenesis of leprosy and assessed their immunoregulatory effects on Tregs and effector T cells. We found an increased frequency of Bregs and increased expression of their immune modulatory molecules (IL-10, FoxP3, and PDL-1) in leprosy patients. The potential immunoregulatory mechanism of Bregs was also investigated using MACS sorted Teff (CD4CD25) and Treg (CD4CD25) cells were cocultured with Bregs to elucidate the effects of Bregs on effector T and regulatory T cells. Cell coculture results showed that purified Bregs cells from leprosy patients convert CD4CD25 cells into CD4CD25 cells. Cell coculture experiments also demonstrated that leprosy derived IL-10 producing Bregs enhance FoxP3 and PD-1 expression in Tregs and enhanced Tregs activity. Blocking of IL-10 receptor confirmed that IL-10 producing Breg has immunomodulatory effect on Tregs and effector T cells as effector T cells are not converted into Tregs and enhanced expression of FoxP3 and PD-1 was not observed on Tregs. Collectively, these findings demonstrate that IL-10 producing Breg cells play an important mechanism in controlling the immunopathogenesis of leprosy and have an immunomodulatory effect on Tregs and effector T cells. Our findings may pave way for novel targets of IL-10 producing Bregs for immunotherapy in leprosy patients.</p>
},
  year = {2018},
  journal = {Frontiers in immunology},
  volume = {9},
  pages = {1636},
  issn = {1664-3224},
  url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065098/pdf/fimmu-09-01636.pdf},
  doi = {10.3389/fimmu.2018.01636},
  language = {eng},
}