@article{31456,
  keywords = {Real Time PCR, Phagocytosis, Nutrient, Mycbacterium leprae, leprosy, Acanthamoeba sp},
  author = {Paling S and Wahyuni R and Winarni D and Astari L and Adriaty D and Agusni I and Izumi S},
  title = {Acanthamoeba SP.S-11 Phagocytotic activity on Mycobacterium Leprae in different nutrient conditions.},
  abstract = {<h4>Background: M<em>ycobacterium leprae</em> (<em>M. leprae</em>) is a pathogenic bacterium that causes leprosy. The presence of <em>M. leprae</em> in the environment is supported by microorganisms that act as the new host for <em>M. leprae</em>. <em>Acanthamoeba</em>'s potential to be a host of <em>M. leprae</em> in the environment. <em>Acanthamoeba</em> sp. is <em>Free Living Amoeba</em> (FLA) that classified as holozoic, saprophytic, and saprozoic. The existence of nutrients in the environment influence <em>Acanthamoeba</em> ability to phagocytosis or pinocytosis. This study is aimed to determine <em>Acanthamoeba</em> sp.S-11 phagocytic activity to <em>Mycobacterium leprae</em> (<em>M. leprae</em>) which cultured in non-nutrient media and riched-nutrient media.</h4>

<h4>Materials and Methods: This research conducted by culturing <em>Acanthamoeba</em> sp.S-11 and <em>M. leprae</em> on different nutrient media conditions. <em>M. leprae</em> intracellular DNA were isolated and amplified by <em>M. leprae</em> specific primers through Real Time PCR (Q-PCR).</h4>

<h4>Result: The results showed that <em>Acanthamoeba</em> co-cultured on non-nutrient media were more active to phagocyte <em>M. leprae</em> than on rich-nutrient media.</h4>

<h4>Conclusion: The use of non-nutrient media is recommended to optimize <em>Acanthamoeba</em> sp. phagocytic activity to <em>M. leprae.</em></h4>
},
  year = {2018},
  journal = {African journal of infectious diseases},
  volume = {12},
  pages = {44-48},
  issn = {2006-0165},
  url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876777/pdf/AJID-12-44.pdf},
  doi = {10.2101/Ajid.12v1S.5},
  language = {eng},
}