@article{2676,
  keywords = {Animals, Base Sequence, Chromosome Mapping, Genome, Bacterial, Humans, leprosy, Molecular Sequence Data, Mycobacterium leprae},
  author = {Eiglmeier K and Simon S and Garnier T and Cole S T},
  title = {The integrated genome map of Mycobacterium leprae.},
  abstract = {<p>The integrated map of the Mycobacterium leprae genome unveiled for the first time the genomic organization of this obligate intracellular parasite. Selected cosmid clones, isolated from a genomic library created in the cosmid vector Lorist6, were identified as representing nearly the complete genome and were subsequently used in the M. leprae genome sequencing project. Now a new version of the integrated map of M. leprae can be presented, combining the mapping results from the Lorist6 cosmids with data obtained from a second genomic library constructed in an Escherichia coli-mycobacterium shuttle cosmid, pYUB18. More than 98% of the M. leprae genome is now covered by overlapping large insert genomic clones representing a renewable source of well defined DNA segments and a powerful tool for functional genomics.</p>},
  year = {2001},
  journal = {Leprosy review},
  volume = {72},
  pages = {462-9},
  month = {2001 Dec},
  issn = {0305-7518},
  url = {http://leprev.ilsl.br/pdfs/2001/v72n4/pdf/v72n4a09.pdf},
  language = {eng},
}