@article{2420,
  keywords = {Antigens, Bacterial, Cell Line, Glycolipids, Humans, Leukocytes, Mononuclear, Mycobacterium leprae, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha},
  author = {Charlab R and Sarno E N and Chatterjee D and Pessolani M C},
  title = {Effect of unique Mycobacterium leprae phenolic glycolipid-I (PGL-I) on tumour necrosis factor production by human mononuclear cells.},
  abstract = {<p>Mycobacterium leprae cell wall-associated components are found in large amounts in the tissues of leprosy patients, particularly those at the lepromatous pole. Among these molecules, the phenolic glycolipid-I (PGL-I), unique to M. leprae, has been involved in the selective anergy observed in the lepromatous patients. Armadillo-derived M. leprae retains only a small proportion of the total PGL-I found in infected tissues. Therefore, the addition of PGL-I to M. leprae in vitro is important for a better understanding of M. leprae effects in vivo. We have studied the influence of PGL-I on TNF production by normal human peripheral blood mononuclear cells (PBMC) and by a human monocytic leukaemia cell line (THP-1) following stimulation with killed M. leprae. PGL-I alone did not induce TNF secretion by PBMC, but when associated with a sub-optimal dose of armadillo-derived M. leprae increased the release of this cytokine. In agreement with these results, M. leprae-exposed THP-1 cells did not secrete detectable levels of TNF unless PGL-I was simultaneously added to the culture. This increase in TNF production suggests that PGL-I plays a role in the induction of TNF during the natural infection. In addition, the modulatory effect of PGL-I on TNF release by THP-1 cells reinforces that monocytes are one of the possible targets of this molecule.</p>},
  year = {2001},
  journal = {Leprosy review},
  volume = {72},
  pages = {63-9},
  month = {2001 Mar},
  issn = {0305-7518},
  url = {http://leprev.ilsl.br/pdfs/2001/v72n1/pdf/v72n1a10.pdf},
  language = {eng},
}