@article{23014,
  keywords = {Animals, B7-1 Antigen, Cell Line, Cytokines, Diamines, Flow Cytometry, Gene Expression Regulation, Humans, Inflammation, Interleukin-10, Leukocytes, Mononuclear, Macrophages, Mice, Reverse Transcriptase Polymerase Chain Reaction, RNA Processing, Post-Transcriptional, Thalidomide},
  author = {Mazzoccoli L and Cadoso S and Amarante G and Souza MV N and Domingues R and Machado M and Almeida MV and Teixeira H},
  title = {Novel thalidomide analogues from diamines inhibit pro-inflammatory cytokine production and CD80 expression while enhancing IL-10.},
  abstract = {<p>Thalidomide is used to treat a variety of diseases including erythema nodosum leprosum, an inflammatory complication of leprosy. However, this drug has severe teratogenic activity and novel thalidomide analogues might be used to treat diseases without this severe side effect. A series of diamine compounds containing two hydrolyzed phthalimide units were chosen as analogues of thalidomide and evaluated regarding their capacity to regulate the production of molecules involved in inflammatory responses. TNF-α, IL-12 and IL-10 production, and the expression of CD80 and CD86 were investigated in LPS plus IFN-γ-stimulated J774A.1 cells by ELISA and flow cytometry, respectively. The expression of TNF-α and IL-10 mRNA was analyzed by real time RT-PCR. TNF-α, IL-6, IFN-γ, CXCL9 and CXCL10 production by human peripheral blood mononuclear cells (PBMC) were evaluated by flow cytometry. Compounds 3, 6 and 9 greatly inhibited TNF-α and IL-12 production while enhancing IL-10. In addition, CD80 expression was inhibited, but not CD86. The compounds inhibited TNF-α production by PBMC more than thalidomide and also had an inhibitory effect on the production of IL-6, IFN-γ, CXCL9 and CXCL10. Levels of mRNA for TNF-α were reduced after treatment with the compounds, suggesting post- transcriptional effects. The compounds had no effect on cell viability. Our results indicate that the novel diamine compounds 3, 6 and 9 inhibit critical pro-inflammatory cytokines and stimulate IL-10, which make them attractive candidate drugs for the treatment of certain inflammatory conditions and cancer.</p>},
  year = {2012},
  journal = {Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie},
  volume = {66},
  pages = {323-9},
  month = {2012 Jul},
  publisher = {Masson Pub. USA, Inc. },
  address = {New York},
  issn = {1950-6007},
  doi = {10.1016/j.biopha.2012.05.001},
  language = {eng},
}