@article{22171,
  keywords = {Antigens, Bacterial, Biological Assay, Biomarkers, Buffers, Cytokines, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, Immunity, Humoral, Interferon-gamma, Interleukin-10, leprosy, Mycobacterium Infections, Reference Values, Sensitivity and Specificity},
  author = {Corstjens P and Dood CJ and Ploeg-van Schip JJ and Wiesmeijer K and Riuttamäki T and Meijgaarden K and Spencer JS and Tanke HJ and Ottenhoff T and Geluk A},
  title = {Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.},
  abstract = {<p><b>OBJECTIVE: </b>The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.</p><p><b>DESIGN AND METHODS: </b>A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.</p><p><b>RESULTS: </b>Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.</p><p><b>CONCLUSIONS: </b>The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.</p>},
  year = {2011},
  journal = {Clinical biochemistry},
  volume = {44},
  pages = {1241-6},
  month = {2011 Oct},
  issn = {1873-2933},
  url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177995/pdf/nihms310566.pdf},
  doi = {10.1016/j.clinbiochem.2011.06.983},
  language = {eng},
}