@article{19407,
  keywords = {Clone Cells, Enzyme-Linked Immunosorbent Assay, Humans, Immunophenotyping, Interferon-gamma, Interleukin-13, Interleukin-4, Interleukin-5, Interleukins, leprosy, Mycobacterium leprae, Skin, Statistics, Nonparametric, Th1 Cells, Th2 Cells, Tumor Necrosis Factor-alpha},
  author = {Verhagen C E and Pouw Kraan T C and Buffing A A and Chand M A and Faber W R and Aarden L A and Das P K},
  title = {Type 1- and type 2-like lesional skin-derived Mycobacterium leprae-responsive T cell clones are characterized by coexpression of IFN-gamma/TNF-alpha and IL-4/IL-5/IL-13, respectively.},
  abstract = {<p>In an earlier study, we generated a large number of Mycobacterium leprae-responsive and M. leprae-nonresponsive T cell clones (TCC) from the lesional skin of immunologic unstable borderline leprosy patients. In that study, we divided TCC into type 1- and type 2-like on the basis of their IFN-gamma and IL-4 expression. To explore whether other cytokines are coproduced along with IFN-gamma and IL-4, we investigated the secretion of a panel of other cytokines (TNF-alpha, IL-5, IL-6, IL-10, and IL-13) by a large number of these TCC. Upon analysis of 139 M. leprae-responsive TCC, we observed a positive correlation in the coproduction of IFN-gamma/TNF-alpha (r = 0.81), and in that of IL-4/IL-5 (r = 0.83), IL-4/IL-13 (r = 0.80), and IL-5/IL-13 (r = 0.82). Polarized type 1-like TCC produced dominantly IFN-gamma/TNF-alpha, and polarized type 2-like TCC predominantly IL-4/IL-5/IL-13. Most type 0-like TCC produced both sets of cytokines. In contrast, type 1- and type 2-like subsets of M. leprae-nonresponsive TCC (n = 58) did not show the same coexpression of these cytokines. Furthermore, when the differential expression of a broad panel of cytokines by individual M. leprae-responsive TCC is considered, it appeared that additional phenotypes could be recognized. These results suggested that distinct isotypes of type 1- and type 2-like T cells, based on the secretion of a panel of cytokines, may reflect M. leprae-specific characteristics.</p>},
  year = {1998},
  journal = {Journal of immunology (Baltimore, Md. : 1950)},
  volume = {160},
  pages = {2380-7},
  month = {1998 Mar 01},
  issn = {0022-1767},
  url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1866584/pdf/1720.pdf},
  language = {eng},
}