@article{19153,
  keywords = {Adolescent, Adult, Antibodies, Bacterial, Antibodies, Monoclonal, Antigen-Antibody Complex, Antigens, Bacterial, Chaperonin 10, Enzyme-Linked Immunosorbent Assay, Erythema Nodosum, Glycolipids, Humans, Immunoglobulin G, Immunoglobulin M, Leprosy, lepromatous, Middle Aged, Polyethylene Glycols, Recombinant Proteins},
  author = {Rojas R E and Demichelis S O and Sarno E N and Segal-Eiras A},
  title = {IgM anti-phenolic glycolipid I and IgG anti-10-kDa heat shock protein antibodies in sera and immune complexes isolated from leprosy patients with or without erythema nodosum leprosum and contacts.},
  abstract = {<p>The aim of the present work was to evaluate the levels of anti-PGL-I and anti-10-kDa heat shock protein antibodies in serum and immune complexes isolated from leprosy patients, convivients and controls. Leprosy patients with erythema nodosum leprosum or without it were included and a comparative study was done to investigate intergroup differences. Immune complexes were precipitated from serum by polyethylene glycol 3.5%; antibody levels were measured in sera and in dissociated immune complexes by ELISA. Serum antibody levels were then correlated with immune complex-associated antibody levels. The results showed that the erythema nodosum leprosum group differed from controls, contacts and non-erythema patients in their immune complex levels. IgM anti-PGL-I and IgG anti-10-kDa heat shock protein antibodies were constituents of the immune complexes in patients with erythema nodosum leprosum, who exhibited a significant difference in their immune complex composition compared with controls, contacts and non-erythema patients; while free antibody levels (anti-PGL-I and anti-10-kDa) did not differentiate between erythema and non-erythema patients, the measurement of immune complex-associated antibodies demonstrated a significant difference between the two clinical conditions. Furthermore, the measurement of immune complex-associated anti-PGL-I IgM made it possible to differentiate between contacts and controls. The significance of these results is discussed.</p>},
  year = {1997},
  journal = {FEMS immunology and medical microbiology},
  volume = {19},
  pages = {65-74},
  month = {1997 Sep},
  issn = {0928-8244},
  doi = {10.1111/j.1574-695X.1997.tb01073.x},
  language = {eng},
}