@article{18534,
  keywords = {3T3 Cells, Animals, Antigen-Presenting Cells, Antigens, Bacterial, Cells, Cultured, Formaldehyde, HLA-DR Antigens, Heat-Shock Proteins, Humans, Interferon-gamma, Keratinocytes, Lymphocyte Activation, Mice, Mycobacterium leprae, Polymers, Recombinant Proteins, T-Lymphocytes, Helper-Inducer},
  author = {Mutis T and De Bueger M and Bakker A and Ottenhoff T H},
  title = {HLA class II+ human keratinocytes present Mycobacterium leprae antigens to CD4+ Th1-like cells.},
  abstract = {<p>In a variety of inflammatory skin diseases like leprosy, keratinocytes (KC) are induced to express MHC class II molecules and may therefore serve as antigen-presenting cells (APC) for MHC class II restricted T cells infiltrating the lesions. However, KC have been thought to be improper APC for MHC class II restricted T cells and to drive T cells into an anergic rather than into an activation state. We evaluated this issue in relation to leprosy and tested whether HLA-DR+ KC could present M. leprae antigens to well-defined, CD4+, cytotoxic as well as proliferative, Th1-like cell clones. Using a recently developed sensitive assay system which employs intact layers of basal KC as APC we found that most T-cell clones (6/8) lysed HLA-DR+ KC pulsed with M. leprae antigens. KC were only recognized after induction of HLA-DR expression by IFN-gamma, in an antigen-specific and HLA class II restricted manner. All T-cell clones tested also showed significant proliferation and IFN-gamma production in response to M. leprae antigens presented by HLA-DR+ KC, arguing against a KC dependent anergizing effect on T cells. Thus, HLA class II+ KC can function as proper APC for HLA class II restricted CD4+ Th 1-like cells. It seems therefore possible that antigen presentation by KC contributes to the local cell-mediated immune responses in DTH lesions.</p>},
  year = {1993},
  journal = {Scandinavian journal of immunology},
  volume = {37},
  pages = {43-51},
  month = {1993 Jan},
  issn = {0300-9475},
  doi = {10.1111/j.1365-3083.1993.tb01663.x},
  language = {eng},
}