@article{18409,
  keywords = {Animals, Cells, Cultured, Culture Media, Humans, leprosy, Mice, Mycobacterium leprae, Skin},
  author = {Bhatia V N and Thawani G},
  title = {Observations on attempted leprosy cultures in two media.},
  abstract = {<p>Suspensions of skin tissue material collected from lepromatous leprosy patients and material from mouse foot-pad harvests were inoculated into two media, viz., a biphasic medium and a minimal basal medium. The cultures were incubated at 37 degrees C and 15 degrees C. Small oval (or round) cells appeared in these cultures around the tenth day along with a few cystic structures; and they increased in number later, reaching the maximum around six-seven weeks. The above cells appeared acid-fast in some cultures and some of them appeared to split into pairs of acid-fast bacilli. The cells were most often seen in the biphasic medium at 37 degrees C. The identity of these structures is not known at this stage.</p>},
  year = {1993},
  journal = {Indian journal of leprosy},
  volume = {65},
  pages = {163-71},
  month = {1993 Apr-Jun},
  issn = {0254-9395},
  language = {eng},
}