@article{18384,
  keywords = {Animals, Dapsone, Drug Resistance, Microbial, Female, Humans, leprosy, Male, Mice, Mice, Inbred BALB C, Mycobacterium leprae, Rifampin},
  author = {Gonzalez A B and Maestre J L and Hernandez O and Columbié Y and Atrio N and Martin M and Fernández A M and Rodríguez J},
  title = {Survey for secondary dapsone and rifampicin resistance in Cuba.},
  abstract = {<p>A total of 1211 Cuban multibacillary leprosy patients treated for at least 5 years were clinically and bacteriologically examined. They were being treated according to a 2-phase monotherapy regimen with RMP first and DADDS afterwards. On skin-smear examination 50 patients were found positive, of which 9 showed a BI of 3+ or higher at any site. With regard to the clinical status the only cases found with clinical signs of relapse were 5 out of 7 long-standing patients with BI of 4+ and 5+. A 6th patient of this high BI group who showed a good clinical condition, except for a heavy infiltration of both earlobes, was receiving a second RMP course when examined and biopsied for this research. These 9 patients were biopsied and susceptibility tests to RMP and DDS performed. The results showed that in 1 case the Mycobacterium leprae were resistant to both drugs; the organisms from 2 other patients were susceptible to RMP but low-grade resistant to DDS. Those from another patient were susceptible to RMP and fully resistant to DDS. In 3 other cases the bacilli did not multiply in any of the mice but 1 of these strains was from the patient taking a second RMP course, therefore this strain might also be susceptible to RMP and resistant to DDS. In the last 2 cases multiplication was only observed in 2 of the controls and in 1 of the 0.0001% DDS treated mice; therefore, these experiments were not conclusive, and the AFB recovered were inoculated into fresh mice to repeat the tests but these failed to multiply.</p>},
  year = {1993},
  journal = {Leprosy review},
  volume = {64},
  pages = {128-35},
  month = {1993 Jun},
  issn = {0305-7518},
  url = {http://leprev.ilsl.br/pdfs/1993/v64n2/pdf/v64n2a06.pdf},
  doi = {10.5935/0305-7518.19930015},
  language = {eng},
}