@article{18269,
  keywords = {Animals, Antibodies, Anti-Idiotypic, Antibodies, Bacterial, Antibodies, Monoclonal, Antigens, Bacterial, Biomarkers, Glycolipids, Humans, Hybridomas, Mice, Mice, Inbred BALB C, Mycobacterium leprae, Trisaccharides},
  author = {Wang D and Jiang Z and Mu J and Li M and Wang B},
  title = {[Development of hybridomas secreting monoclonal anti-idiotypic antibodies that bear the internal image of the terminal trisaccharide of PGL-I].},
  abstract = {<p>Two hybridomas designated as F7B7 and F7B9 secreting monoclonal anti-idiotypic antibodies against terminal trisaccharide of PGL-I were developed by fusion of SP2/0 cells and spleen cells of BALB/c mouse immunized with mouse monoclonal anti-trisaccharide of PGL-I (MAb1-E10F1). To characterize the F7B7, the following results were obtained. First, F7B7 reacted with MAb1-E10F1 specifically. Secondly, the cross ELISA neutralizing tests gave positive results. The binding of anti-trisaccharide positive serum with trisaccharide (contained in semi-synthetic antigen, NT-O-BSA) was inhibited F7B7 and the degree of inhibition showed dose-dependent manner. The binding of anti-trisaccharide positive serum with F7B7 was inhibited by NT-O-BSA and the degree of inhibition also showed dose-dependent manner. It was concluded that the hybridoma F7B7 is able to secrete monoclonal anti-idiotypic antibodies that bear the internal image of trisaccharide of PGL-I. The potentials and advantages of monoclonal anti-idiotypic antibody F7B7 as surrogate antigen in the serodiagnosis of leprosy have been discussed.</p>},
  year = {1993},
  journal = {Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao},
  volume = {24},
  pages = {250-3},
  month = {1993 Sep},
  issn = {0257-7712},
  language = {chi},
}