@article{18206,
  keywords = {Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Proteins, Child, Child, Preschool, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, leprosy, Middle Aged, Mycobacterium bovis, Mycobacterium leprae, Sensitivity and Specificity, Tuberculosis},
  author = {Vikerfors T and Olcén P and Wiker H and Watson J D},
  title = {Serological response in leprosy and tuberculosis patients to the 18-kDa antigen of Mycobacterium leprae and antigen 85B of Mycobacterium bovis BCG.},
  abstract = {<p>Two enzyme-linked immunosorbent assays (ELISAs) for the detection of leprosy antibodies were developed. These assays were based on the recombinant 18-kDa protein of Mycobacterium leprae and the 85B antigen of M. bovis. Sera from leprosy and tuberculosis patients were analyzed, and a phenolic glycolipid-I (PGL-I) ELISA was used as a reference test. The 18-kDa ELISA reached a sensitivity of 70% on lepromatous leprosy sera. The corresponding results for the 85B and the PGL-I ELISAs were 93% and 96%, respectively. The sensitivity on tuberculoid leprosy sera was 72% for the 18-kDa antigen, 95% for the 85B antigen, and 60% for the PGL-I antigen. The 18-kDa antigen ELISA was found to have crossreactivity to sera from patients with tuberculosis, while the 85B and PGL-I assays had high specificity.</p>
},
  year = {1993},
  journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  volume = {61},
  pages = {571-80},
  month = {1993 Dec},
  issn = {0148-916X},
  url = {http://ila.ilsl.br/pdfs/v61n4a06.pdf},
  language = {eng},
}