@article{18166,
  keywords = {Antibodies, Bacterial, Antigens, Bacterial, Bacterial Proteins, Heat-Shock Proteins, Humans, Immunoglobulin G, leprosy, Molecular Weight, Mycobacterium leprae},
  author = {Launois P and N'Diaye Niang M and Drowart A and Van Vooren J P and Sarthou J L and Lalu T and Millan J and Huygen K},
  title = {IgG response to purified 65- and 70-kDa mycobacterial heat shock proteins and to antigen 85 in leprosy.},
  abstract = {<p>IgG antibody response to mycobacterial heat-shock proteins (hsp) (the 70-kDa antigen from Mycobacterium tuberculosis and M. bovis BCG; the 65-kDa antigen from M. leprae and M. bovis BCG) and to the fibronectin-binding antigen 85 from M. bovis BCG was analyzed in a dot-blot assay in plasma from leprosy patients and their contacts. Most plasma--whatever the status of the subjects--reacted to the hsp 70; 8 of 9 (89%) of paucibacillary patients recognized the 65 mycobacterial hsp but only 2 of 9 (22%) recognized the antigen 85. In contrast, 12 of 12 (100%) of multibacillary patients reacted with the antigen 85 and only 4 of 12 (33%) reacted to the hsp 65 from M. leprae. On the one hand, 7 of 25 (28%) of the lepromin-positive contacts and 2 of 9 (22%) of the lepromin-negative contacts recognized the antigen 85. On the other hand, 11 of 25 (44%) of the lepromin-positive contacts but only 1 of 9 (11%) of the lepromin-negative contacts reacted to the hsp65 from M. leprae. Finally, very few (10%) of the lepromin-positive controls showed a positive reaction to the M. leprae 65-kDa antigen, the BCG 65-kDa antigen, and the 85-kDa antigen of BCG. Thus, differences in binding to the hsp65 from M. leprae and to antigen 85 could be helpful in distinguishing different forms of the disease.</p>
},
  year = {1994},
  journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  volume = {62},
  pages = {48-54},
  month = {1994 Mar},
  issn = {0148-916X},
  url = {http://ila.ilsl.br/pdfs/v62n1a07.pdf},
  language = {eng},
}