@article{17867,
  keywords = {Antigens, Bacterial, Enzyme-Linked Immunosorbent Assay, Humans, Immunity, Cellular, Interferon-gamma, leprosy, Lymphocyte Activation, Mycobacterium leprae, Nepal, Seroepidemiologic Studies, T-Lymphocytes, Thymidine, Tuberculin},
  author = {Weir R E and Morgan A R and Britton W J and Butlin C R and Dockrell H M},
  title = {Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity.},
  abstract = {<p>A whole blood assay is described to measure T cell mediated immune responses to leprosy and provide an alternative to the conventional lymphocyte transformation test. Optimal conditions were defined for the whole blood assay, and interferon-gamma measurement was found to be a more sensitive way of measuring responses than tritiated thymidine incorporation. The assay was shown to be useful for investigating responses to a range of leprosy antigens. A whole blood assay has the advantages of being quick, simple and requiring only a small volume of blood, making it more appropriate as an immuno-epidemiological field test in leprosy endemic areas.</p>},
  year = {1994},
  journal = {Journal of immunological methods},
  volume = {176},
  pages = {93-101},
  month = {1994 Nov 10},
  issn = {0022-1759},
  doi = {10.1016/0022-1759(94)90353-0},
  language = {eng},
}