@article{17857,
  keywords = {Animals, Bacterial Vaccines, DNA, Bacterial, Mycobacterium tuberculosis, Transfection, Tuberculosis},
  author = {Lowrie D B and Tascon R E and Colston M J and Silva C L},
  title = {Towards a DNA vaccine against tuberculosis.},
  abstract = {<p>Expression of the gene for a single mycobacterial antigen (Mycobacterium leprae hsp65) in adult Balb/c mice resulted in substantial cell-mediated protection against challenge with M. tuberculosis. CD4 and CD8 T cells cloned from spleens of such immunized mice passively transferred protection to non-immunized mice, and CD8 cells selectively lysed macrophages infected with M. tuberculosis. Three modes of expressing the gene have been tested: (1) expression from a retroviral vector (pZIPNeoSV) in implanted J774 tumour cells, (2) expression from the same vector via bone marrow cells transfected in vitro and used to reconstitute irradiated mice, and (3) in a preliminary experiment, from CMV immediate-early and hydroxymethylglutaryl Co-A reductase promoters injected as plasmid DNA into muscle.</p>},
  year = {1994},
  journal = {Vaccine},
  volume = {12},
  pages = {1537-40},
  month = {1994 Dec},
  issn = {0264-410X},
  doi = {10.1016/0264-410x(94)90080-9},
  language = {eng},
}