@article{17393,
  keywords = {Base Sequence, DNA Primers, Evaluation Studies as Topic, Humans, leprosy, Molecular Sequence Data, Mycobacterium leprae, polymerase chain reaction, RNA, Bacterial, RNA, Ribosomal, 16S, Species Specificity},
  author = {Richter E and Duchrow M and Schlüter C and Hahn M and Flad H D and Gerdes J},
  title = {Detection of Mycobacterium leprae by three-primer PCR.},
  abstract = {<p>Recently, polymerase chain reaction has been introduced for the species-specific assessment of Mycobacterium leprae (1). To avoid Southern blotting techniques using radioactively labelled oligonucleotide probes, the aim of this study was to establish a three primer-based single-step PCR technique. Using primers designed for this purpose we amplified a part of the gene encoding for the 16S ribosomal RNA of slowly growing mycobacteria. Due to the species-specific antisense primer a second, smaller fragment specific for M. leprae was amplified. Our results show that the employment of a second antisense primer in the PCR may be a substitution for Southern blot hybridization.</p>},
  year = {1994},
  journal = {Immunobiology},
  volume = {191},
  pages = {351-3},
  month = {1994 Oct},
  issn = {0171-2985},
  doi = {10.1016/S0171-2985(11)80440-7},
  language = {eng},
}