@article{17208,
  keywords = {Animals, Antibodies, Antibody Specificity, Arthritis, Rheumatoid, Collagen, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Humans, leprosy, Lupus Erythematosus, Systemic, Rabbits},
  author = {Meghlaoui A and Herbage D and Huc A and Monier J C},
  title = {[Detection of anti-collagen type I and II antibodies by an immunoenzymatic technique (ELISA): results in rheumatoid arthritis, systemic lupus erythematosus and leprosy (author's transl)].},
  abstract = {<p>An attempt was made to detect antibodies against type I and/or II collagen in sera from patients with rheumatoid arthritis, systemic lupus erythematosus (SLE) and leprae. This study was performed with an immunoenzymatic technique: ELISA (enzyme-linked immunosorbent assay). The following steps were performed: bovine collagen type I or II was adsorbed on glass beads; possible free sites were saturated by incubating the beads with sheep serum; then, the antibodies specifically bound to collagen were detected by a peroxidase-labelled anti-immunoglobulin; the immune complexes at the surface of the beads were revealed by a substrate specific for peroxidase and of great stability: Trinder's reactive. Using conditions previously shown to be optimal, the prevalence of anti-collagen antibodies was as follows. In patients with lepromatous leprae the percentages of positive sera against collagen type I and II were 40% and 44%, respectively; in patients with tuberculoid leprae the percentages were lower: 10% and 30%, respectively. Ten per cent of the SLE patients had antibodies against collagen type I, half of the prevalence noted for anti-collagen type II antibodies (20%). Finally, 13.6% of the patients with rheumatoid arthritis had antibodies against collagen type I, a percentage very similar to that of the patients with anti-collagen type II antibodies (14.6%).</p>},
  year = {1981},
  journal = {Annales d'immunologie},
  volume = {132C},
  pages = {287-305},
  month = {1981 May-Jun},
  issn = {0300-4910},
  language = {fre},
}