@article{1693,
  keywords = {Adult, Aged, Antibodies, Monoclonal, B-Lymphocytes, B7-1 Antigen, CD28 Antigens, Cell Division, Female, Humans, Interferon-gamma, Leprosy, lepromatous, Leukocytes, Mononuclear, Male, Middle Aged, Phytohemagglutinins, T-Lymphocytes},
  author = {Fafutis-Morris M and Guillen-Vargas C M and Navarro-Fierros S and Alfaro-Bustamante F and Zaitzeva-Petrovna G and Daneri-Navarro A and Santoscoy-Tovar L and Armendariz-Borunda J},
  title = {Addiction of anti-CD28 antibodies restores PBMC proliferation and IFN-gamma production in lepromatous leprosy patients.},
  abstract = {<p>During antigen recognition, T lymphocytes are primed by a physical interaction with antigen-presenting cells (APC). At least two signals are needed to activate T cells. One is provided by T cell receptor (TCR)/CD3 in the context of the mayor histocompatibility complex (MHC), and another signal is mediated by antigen-independent molecules, that is T cell membrane-bound CD28 and its specific ligand B7-1 (CD80) present in APC. Both signals trigger a series of metabolic events initiating right at the cell membrane and ending with activation and proliferation of T cells as well as specific cytokines synthesis. Our main goal was to determine whether deficiency in interferon-gamma (IFN-gamma) production shown by peripheral blood mononuclear cells (PBMC) from lepromatous leprosy (LL) patients, could be overcome by reconstituting in vitro the appropriate signals (by means of addition of anti-CD28 and anti-CD80 monoclonal antibodies). We also determined the stimulation index (SI) in the same PBMC. Our results demonstrated no significant differences in CD80 expression monocytes and B lymphocytes from LL patients when compared with healthy subjects. Nonetheless, CD28 expression significantly decreased in lymphocytes from LL patients (p < 0.01). Regarding IFN-gamma levels and SI, LL-PBMC failure before mitogenic stimuli could be reversed by further incubation with anti-CD28 antibody, but stimulation by specific antigen of Mycobacterium leprae was not changed. Addition of anti-CD80 antibody significantly increased IFN-gamma levels in phytohemagglutinin (PHA)-stimulated PBMC, although proliferation deficiency persisted. Cells stimulated with specific antigen did not modify either their proliferation or IFN-gamma levels.</p>},
  year = {1999},
  journal = {Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research},
  volume = {19},
  pages = {1237-43},
  month = {1999 Nov},
  issn = {1079-9907},
  doi = {10.1089/107999099312894},
  language = {eng},
}