@article{16545,
  keywords = {Animals, Color, Humans, leprosy, Methods, Mice, Mycobacterium, Rats, Rosaniline Dyes, Staining and Labeling},
  author = {Harada K},
  title = {A modified allochrome procedure for demonstrating mycobacteria in tissue sections.},
  abstract = {<p>A modified allochrome staining procedure is presented as being the most reliable and sensitive method for demonstrating mycobacteria in tissue sections. The technic is as follows: Deparaffinize formalin fixed sections, oxidize in 10% periodic acid for 24 hours, differentiate in 1% HCl-70% ethanol, stain in Weigert's iron hematoxylin nuclear stain, and counterstain in picro-methyl glue. Mycobacteria stained brilliant red in contrast with the allochrome-stained background tissues, and apparently otherwise chromophobic bacilli are demonstrated.</p>
},
  year = {1977},
  journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association},
  volume = {45},
  pages = {49-51},
  month = {1977 Jan-Mar},
  issn = {0148-916X},
  url = {http://ila.ilsl.br/pdfs/v45n1a07.pdf},
  language = {eng},
}