@article{16012,
  keywords = {Blood Proteins, Cell Adhesion, Cells, Cultured, Dose-Response Relationship, Immunologic, Female, Histocompatibility Antigens Class II, Humans, Immune Tolerance, leprosy, Lymphokines, Male, Monocytes, Solubility, Suppressor Factors, Immunologic, T-Lymphocytes},
  author = {Sathish M and Bhutani L K and Sharma A K and Nath I},
  title = {Monocyte-derived soluble suppressor factor(s) in patients with lepromatous leprosy.},
  abstract = {<p>Peripheral blood monocytes from polar lepromatous leprosy (LL) patients were unable to support Mycobacterium leprae-induced in vitro lymphoproliferation of HLA-D-matched T cells from tuberculoid leprosy subjects, whereas those from responder individuals were able to do so. Monocyte-rich adherent cells from untreated LL patients released de novo soluble factors which inhibited antigen-induced lymphoproliferation to a greater extent and mitogenic responses to a lesser extent. Suppressive activity varied in different LL patients. However, the degree of suppression was similar in soluble factors obtained de novo and after treatment of adherent cells with heat-killed and freshly extracted, cryopreserved M. leprae. Treated patients showed less inhibition with de novo released soluble factors (27 +/- 7.7%) as compared to parallel soluble factors obtained after antigen treatment (44 +/- 4.8%) or with de novo soluble factors from untreated LL patients (62 +/- 14.2%). Similar supernatants from tuberculoid individuals showed no or insignificant effects on antigen-induced lymphoproliferation. The suppressive activity of LL soluble factors was produced for up to 72 h, was heat stable at 56 degrees C for 30 min, was indomethacin resistant, and resided in the greater than 25,000 molecular weight fraction.</p>},
  year = {1983},
  journal = {Infection and immunity},
  volume = {42},
  pages = {890-9},
  month = {1983 Dec},
  issn = {0019-9567},
  language = {eng},
}