@article{15703,
  keywords = {Antigens, Bacterial, Electrophoresis, Polyacrylamide Gel, Humans, Immunoenzyme Techniques, leprosy, Mycobacterium leprae},
  author = {Klatser P R and Rens M M and Eggelte T A},
  title = {Immunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera.},
  abstract = {<p>In this study the SDS-polyacrylamide gel electrophoresis immunoperoxidase (SGIP) assay was used for characterizing the antigenic components of Mycobacterium leprae using patients' sera. This technique involved the separation of mycobacterial sonicates on SDS-polyacrylamide gels, longitudinal sectioning of the gels, incubation with patients' sera and visualization of the antigen-antibody complexes by the indirect immunoperoxidase technique. A number of antigens present in M. leprae sonicates were recognized by leprosy patients' sera, some of which were seen in other mycobacteria as well. Antibody binding to a 33 kD antigen, present in both M. leprae and BCG sonicates, was reduced only in the latter after 6 months of multiple drug treatment of one patient. It is suggested that this is a common mycobacterial antigen with one or more M. leprae specific determinants. Several antigens were identified only in M. leprae sonicates, only by leprosy patients: a 12, 22, 28, 36, 41 and 86 kD component. These antigens lost their antigenicity after trypsin treatment, but were heat stable. Such M. leprae specific antigens may be useful for immunodiagnosis.</p>},
  year = {1984},
  journal = {Clinical and experimental immunology},
  volume = {56},
  pages = {537-44},
  month = {1984 Jun},
  issn = {0009-9104},
  language = {eng},
}