@article{11883, keywords = {Antigens, Bacterial, Glycolipids, Humans, Immunosorbent Techniques, leprosy, Membranes, Artificial, Mycobacterium leprae, Polymers, Sulfones}, author = {Young D B and Fohn M J and Buchanan T M}, title = {Use of a polysulfone membrane support for immunochemical analysis of a glycolipid from Mycobacterium leprae.}, abstract = {

Polysulfone membranes have been used as a solid support for chromatography and immunoblotting of phenolic glycolipid I from Mycobacterium leprae. These membranes have an advantage over other supports such as nitrocellulose and silica gel in that very little non-specific background binding of antibodies occurs and assays can readily be carried out with IgM antibodies from human sera. An example of use of the polysulfone chromatography system for detection of phenolic glycolipid I in sera from leprosy patients is described.

}, year = {1985}, journal = {Journal of immunological methods}, volume = {79}, pages = {205-11}, month = {1985 May 23}, issn = {0022-1759}, doi = {10.1016/0022-1759(85)90100-0}, language = {eng}, }