@article{11325,
  keywords = {Animals, Antibodies, Antigens, Bacterial, Color, Glycolipids, Humans, Immunoenzyme Techniques, Immunoglobulin M, leprosy, Mice},
  author = {Kumar S and Moudgil K D and Band A H and Naraynan P R and Gupta S K and Sharma A K and Talwar G P},
  title = {A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I in sera from leprosy patients.},
  abstract = {<p>A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.</p>},
  year = {1986},
  journal = {Indian journal of leprosy},
  volume = {58},
  pages = {185-90},
  month = {1986 Apr-Jun},
  issn = {0254-9395},
  language = {eng},
}