@article{11273, keywords = {Antibodies, Bacterial, Antigens, Bacterial, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, Immunoglobulin M, leprosy, Monitoring, Physiologic, Mycobacterium leprae, Serologic Tests}, author = {Meeker H C and Levis W R and Sersen E and Schuller-Levis G and Brennan P J and Buchanan T M}, title = {ELISA detection of IgM antibodies against phenolic glycolipid-I in the management of leprosy: a comparison between laboratories.}, abstract = {

IgM antibodies to the phenolic glycolipid-I (PGL-I) antigen of Mycobacterium leprae were detected by different ELISA techniques in three laboratories (in New York, Colorado, Seattle, U.S.A.). The agreement on seropositivity and overall correlation between techniques was excellent. A positive linear correlation between the bacterial index (BI) and anti-PGL-I IgM, previously reported by the New York laboratory, was detected by all techniques. The role of erythema nodosum leprosum in decreasing the relationship of BI versus anti-PGL-I IgM was seen by the New York laboratory with sera diluted 1:20 and ABTS substrate solution and by the Colorado laboratory but not by New York with sera at 1:300 and OPD substrate or by the Seattle laboratory.

}, year = {1986}, journal = {International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association}, volume = {54}, pages = {530-9}, month = {1986 Dec}, issn = {0148-916X}, language = {eng}, }