@article{11261,
  keywords = {Antigens, Bacterial, Enzyme-Linked Immunosorbent Assay, Glycolipids, Humans, Immunoelectrophoresis, leprosy, Mycobacterium leprae},
  author = {Kaldany R R and Maasho K and Ohman R and Reitz-Vick D and Britton S and Lefford M J},
  title = {Methods for the detection of a specific Mycobacterium leprae antigen in the urine of leprosy patients.},
  abstract = {<p>Two methods for detecting the phenolic glycolipid, PGL-1, a Mycobacterium leprae-specific molecule, in the urine of leprosy patients are described. Both methods rely on the 100-fold preconcentration of the urine, which can be accomplished by a single-step ultrafiltration procedure. The equivalent of approximately 2.5 micrograms of PGL-1/ml was detected in the urine of LL patients with an inhibition ELISA. The second method, a direct dot-blot assay on nitrocellulose paper, was much simpler and more sensitive. As little as 3 ng of antigen was detected by the dot-blot technique. PGL-1 was detected in the urine of LL patients.</p>},
  year = {1987},
  journal = {Scandinavian journal of immunology},
  volume = {25},
  pages = {37-43},
  month = {1987 Jan},
  issn = {0300-9475},
  doi = {10.1111/j.1365-3083.1987.tb01044.x},
  language = {eng},
}