@article{11179,
  keywords = {Animals, Antigen-Presenting Cells, Cell Separation, Female, Granuloma, Guinea Pigs, HLA-D Antigens, leprosy, Lymph Nodes, Lymphocyte Activation, Macrophages, Male, Peritoneal Cavity, Rosette Formation, T-Lymphocytes},
  author = {Montreewasuwat N and Curtis J and Turk J L},
  title = {Accessory cell function of cells isolated from Mycobacterium leprae-induced granulomas.},
  abstract = {<p>The large cells from Mycobacterium leprae-induced granulomas in guinea pig lymph nodes were separated by Percoll discontinuous density gradient centrifugation and on a fluorescence-activated cell sorter (FACS) using cross-reacting monoclonal antibody to human MHC Class II antigens. Large Percoll-separated cells (83% Class II antigen positive and 52% macrophage-specific antigen positive) and FACS-separated cells are able to act as antigen-presenting cells for T-cell proliferation to PPD. In previous studies, macrophage antigen-positive cells consistently failed to act as accessory cells. This indicates that there is a population of accessory cells which are macrophage antigen negative and MHC Class II antigen positive present in these M. leprae-induced granulomas.</p>},
  year = {1986},
  journal = {Cellular immunology},
  volume = {102},
  pages = {346-54},
  month = {1986 Oct 15},
  issn = {0008-8749},
  doi = {10.1016/0008-8749(86)90428-4},
  language = {eng},
}