@article{10475,
  keywords = {Amino Acid Sequence, Animals, Antigen-Presenting Cells, Antigens, Bacterial, Clone Cells, DNA, Genes, MHC Class II, HLA-D Antigens, HLA-DR Antigens, HLA-DR2 Antigen, Humans, L Cells (Cell Line), leprosy, Mice, Molecular Sequence Data, Mycobacterium leprae, Recombinant Fusion Proteins, T-Lymphocytes},
  author = {Wilkinson D and Vries R R and Madrigal J A and Lock C B and Morgenstern J P and Trowsdale J and Altmann D M},
  title = {Analysis of HLA-DR glycoproteins by DNA-mediated gene transfer. Definition of DR2 beta gene products and antigen presentation to T cell clones from leprosy patients.},
  abstract = {<p>We have used DNA-mediated gene transfer to express HLA class II molecules in mouse L cells for serological, biochemical, and functional analysis. cDNA clones encoding the DR2 beta a and DR2 beta b products of the DR2Dw2 haplotype were subcloned into a mouse Moloney leukemia virus-based expression vector (pJ4) and transfected separately into mouse L cells together with a HLA-DR alpha/pJ4 construct. These transfectants have allowed differential analysis of the two DR2 beta products in a manner normally prohibited by the concomitant expression seen in B cells. Two-dimensional SDS-PAGE analysis of the transfectants defines the more acidic beta chain as the product of the DR2 beta a sequence, and the more basic chain as the product of the DR2 beta b sequence. The LDR2a transfectants present antigen efficiently to M.leprae-specific T cell clones and are capable of presenting synthetic peptide, 65-kD recombinant mycobacterial antigen and M.leprae. Of the DR2Dw2-restricted T cell clones we have tested, all use the DR2 beta a chain as their restriction element. Inhibition studies with mAbs demonstrate the dependence of presentation by the transfectant on class II and CD4, while mAbs against LFA-1, which substantially inhibit presentation by B-lymphoblastoid cell lines, do not inhibit transfectant presentation.</p>},
  year = {1988},
  journal = {The Journal of experimental medicine},
  volume = {167},
  pages = {1442-58},
  month = {1988 Apr 01},
  issn = {0022-1007},
  doi = {10.1084/jem.167.4.1442},
  language = {eng},
}