@article{10199,
  keywords = {Antibodies, Monoclonal, CD4-Positive T-Lymphocytes, Granuloma, Humans, Immunity, Cellular, Immunoenzyme Techniques, Langerhans Cells, Leprosy, lepromatous, Leukocytes, Mycobacterium bovis, Mycobacterium leprae, T-Lymphocytes, Cytotoxic, T-Lymphocytes, Helper-Inducer, T-Lymphocytes, Regulatory},
  author = {Gross A and Weiss E and Tapia F J and Aranzazu N and Gallinoto M E and Convit J},
  title = {Leukocyte subsets in the granulomatous response produced after inoculation with Mycobacterium leprae-BCG in lepromatous patients.},
  abstract = {<p>Leukocyte subsets present in the granulomatous response produced after the inoculation of a mixture of Mycobacterium leprae and BCG in lepromatous leprosy patients were characterized in situ using monoclonal antibodies and an immunoperoxidase technique. The granuloma produced after M. leprae-BCG inoculation showed a distribution pattern similar to tuberculoid granulomas. T lymphocytes bearing the CD8 phenotype (T cytotoxic/suppressor) were sequestered to the periphery of the epithelioid tubercles and T helper-inducer CD4+ lymphocytes were distributed throughout the infiltrate. Langerhans cells CD1+ were increased in the epidermis, and in dermis they were localized mainly in the mantle surrounding the granuloma. Most of the dermal infiltrate produced after the inoculation or M. leprae-BCG expresses the HLA-DR antigen. Similarly, most keratinocytes were also positive to this MHC antigen. The granulomatous response to BCG was similar to the inoculation of a mixture of M. leprae-BCG, however acid-fast bacilla were still present. The inoculation of M. leprae produced a macrophage granuloma with no clearing of the bacilla which resembles the lepromatous leprosy granuloma.</p>},
  year = {1988},
  journal = {The American journal of tropical medicine and hygiene},
  volume = {38},
  pages = {608-12},
  month = {1988 May},
  issn = {0002-9637},
  doi = {10.4269/ajtmh.1988.38.608},
  language = {eng},
}